Although the male has a normal semen, that is, having a good concentration of sperm and these have good mobility and morphology, the sperm can have alterations in their genetic material.
- Repeat abortions.
- FISH altered sperm.
- Increased sperm DNA fragmentation
- Low fecundation rate in previous cycles
- Bad embryo quality in previous cycles.
- Seed samples with a concentration a little below normal.
- Patients treated with chemo or radiotherapy.
The columns of Annexin V is a technique of sperm selection that allows us to eliminate those sperm that have damage to their DNA and therefore have begun a process of apoptosis, that is,cell death.
This technique consists of contacting the semen sample with a series of magnetic beads that have annexin V attached. This annexin V can bind and retain all those sperm that have begun the process of apoptosis, because when a Spermatozoon enters apoptosis has a protein known as phosphatidylserine that will be to which annexin V binds. Subsequently the sample is passedthrough a magnetic column and all sperm that are stuck to annexin V, having a magnetic pellet cannot go through the column and in this way we will collect only those sperm that do not have apoptosis.